Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia
Identifieur interne : 000E44 ( Main/Exploration ); précédent : 000E43; suivant : 000E45Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia
Auteurs : Samy Kasem [Arabie saoudite, Égypte] ; Ibraheem Qasim [Arabie saoudite] ; Ali Al-Hufofi [Arabie saoudite] ; Osman Hashim [Arabie saoudite] ; Ali Alkarar [Arabie saoudite] ; Ali Abu-Obeida [Arabie saoudite] ; Albagir Gaafer [Arabie saoudite] ; Abdelhamid Elfadil [Arabie saoudite] ; Ahmed Zaki [Arabie saoudite] ; Ahmed Al-Romaihi [Arabie saoudite] ; Nasereldeen Babekr [Arabie saoudite] ; Nadr El-Harby [Arabie saoudite] ; Raed Hussien [Arabie saoudite] ; Ali Al-Sahaf [Arabie saoudite] ; Ali Al-Doweriej [Arabie saoudite] ; Faisal Bayoumi [Arabie saoudite] ; Leo L. M. Poon [République populaire de Chine] ; Daniel K. W. Chu [République populaire de Chine] ; Malik Peiris [République populaire de Chine] ; Ranawaka A. P. M. Perera [République populaire de Chine]Source :
- Journal of Infection and Public Health [ 1876-0341 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN viral (génétique), ARN viral (isolement et purification), Adulte d'âge moyen, Animaux, Anticorps antiviraux (sang), Arabie saoudite (épidémiologie), Bovins, Capra (virologie), Chameaux (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Immunoglobuline G (sang), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (transmission), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Mâle, Ovis (virologie), Phylogénie, Réaction de polymérisation en chaine en temps réel, Réservoirs d'agents pathogènes (virologie), Test ELISA, Zoonoses (transmission), Zoonoses (virologie), Zoonoses (épidémiologie), Études transversales.
- MESH :
- génétique : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- sang : Anticorps antiviraux, Immunoglobuline G.
- virologie : Capra, Chameaux, Infections à coronavirus, Ovis, Réservoirs d'agents pathogènes, Zoonoses.
- épidémiologie : Arabie saoudite, Infections à coronavirus, Zoonoses.
- Adulte d'âge moyen, Animaux, Bovins, Humains, Mâle, Phylogénie, Réaction de polymérisation en chaine en temps réel, Test ELISA, Études transversales.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Camelus (virology), Cattle, Coronavirus Infections (epidemiology), Coronavirus Infections (transmission), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Cross-Sectional Studies, Disease Reservoirs (virology), Enzyme-Linked Immunosorbent Assay, Goats (virology), Humans, Immunoglobulin G (blood), Male, Middle Aged, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Phylogeny, RNA, Viral (genetics), RNA, Viral (isolation & purification), Real-Time Polymerase Chain Reaction, Saudi Arabia (epidemiology), Sheep (virology), Whole Genome Sequencing, Zoonoses (epidemiology), Zoonoses (transmission), Zoonoses (virology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- epidemiology : Coronavirus Infections, Saudi Arabia, Zoonoses.
- genetics : Middle East Respiratory Syndrome Coronavirus, RNA, Viral.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus, RNA, Viral.
- transmission : Coronavirus Infections, Zoonoses.
- veterinary : Coronavirus Infections.
- virology : Camelus, Coronavirus Infections, Disease Reservoirs, Goats, Sheep, Zoonoses.
- Animals, Cattle, Cross-Sectional Studies, Enzyme-Linked Immunosorbent Assay, Humans, Male, Middle Aged, Phylogeny, Real-Time Polymerase Chain Reaction, Whole Genome Sequencing.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans.
This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014–2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed.
The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula.
These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS.
Url:
DOI: 10.1016/j.jiph.2017.09.022
PubMed: 28993171
PubMed Central: 7102853
Affiliations:
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Le document en format XML
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Medicine, Kafrelsheikh University, El Geish Street, Kafrelsheikh 33516, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, El Geish Street, Kafrelsheikh 33516</wicri:regionArea><wicri:noRegion>Kafrelsheikh 33516</wicri:noRegion></affiliation></author><author><name sortKey="Qasim, Ibraheem" sort="Qasim, Ibraheem" uniqKey="Qasim I" first="Ibraheem" last="Qasim">Ibraheem Qasim</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Hufofi, Ali" sort="Al Hufofi, Ali" uniqKey="Al Hufofi A" first="Ali" last="Al-Hufofi">Ali Al-Hufofi</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Hashim, Osman" sort="Hashim, Osman" uniqKey="Hashim O" first="Osman" last="Hashim">Osman Hashim</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Alkarar, Ali" sort="Alkarar, Ali" uniqKey="Alkarar A" first="Ali" last="Alkarar">Ali Alkarar</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Abu Obeida, Ali" sort="Abu Obeida, Ali" uniqKey="Abu Obeida A" first="Ali" last="Abu-Obeida">Ali Abu-Obeida</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Gaafer, Albagir" sort="Gaafer, Albagir" uniqKey="Gaafer A" first="Albagir" last="Gaafer">Albagir Gaafer</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Elfadil, Abdelhamid" sort="Elfadil, Abdelhamid" uniqKey="Elfadil A" first="Abdelhamid" last="Elfadil">Abdelhamid Elfadil</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Zaki, Ahmed" sort="Zaki, Ahmed" uniqKey="Zaki A" first="Ahmed" last="Zaki">Ahmed Zaki</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Romaihi, Ahmed" sort="Al Romaihi, Ahmed" uniqKey="Al Romaihi A" first="Ahmed" last="Al-Romaihi">Ahmed Al-Romaihi</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Babekr, Nasereldeen" sort="Babekr, Nasereldeen" uniqKey="Babekr N" first="Nasereldeen" last="Babekr">Nasereldeen Babekr</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="El Harby, Nadr" sort="El Harby, Nadr" uniqKey="El Harby N" first="Nadr" last="El-Harby">Nadr El-Harby</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Hussien, Raed" sort="Hussien, Raed" uniqKey="Hussien R" first="Raed" last="Hussien">Raed Hussien</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Sahaf, Ali" sort="Al Sahaf, Ali" uniqKey="Al Sahaf A" first="Ali" last="Al-Sahaf">Ali Al-Sahaf</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Doweriej, Ali" sort="Al Doweriej, Ali" uniqKey="Al Doweriej A" first="Ali" last="Al-Doweriej">Ali Al-Doweriej</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Bayoumi, Faisal" sort="Bayoumi, Faisal" uniqKey="Bayoumi F" first="Faisal" last="Bayoumi">Faisal Bayoumi</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Animal Resources, Ministry of Environment, Water and Agriculture, 65 King Abdulaziz Road, Riyadh 11195</wicri:regionArea><wicri:noRegion>Riyadh 11195</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Chu, Daniel K W" sort="Chu, Daniel K W" uniqKey="Chu D" first="Daniel K. W." last="Chu">Daniel K. W. Chu</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Perera, Ranawaka A P M" sort="Perera, Ranawaka A P M" uniqKey="Perera R" first="Ranawaka A. P. M." last="Perera">Ranawaka A. P. M. Perera</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Public Health Laboratory Sciences, School of Public Health, The University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Infection and Public Health</title><idno type="ISSN">1876-0341</idno><idno type="eISSN">1876-035X</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Camelus (virology)</term><term>Cattle</term><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (transmission)</term><term>Coronavirus Infections (veterinary)</term><term>Coronavirus Infections (virology)</term><term>Cross-Sectional Studies</term><term>Disease Reservoirs (virology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Goats (virology)</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Male</term><term>Middle Aged</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Phylogeny</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (isolation & purification)</term><term>Real-Time Polymerase Chain Reaction</term><term>Saudi Arabia (epidemiology)</term><term>Sheep (virology)</term><term>Whole Genome Sequencing</term><term>Zoonoses (epidemiology)</term><term>Zoonoses (transmission)</term><term>Zoonoses (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>ARN viral (isolement et purification)</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Arabie saoudite (épidémiologie)</term><term>Bovins</term><term>Capra (virologie)</term><term>Chameaux (virologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (transmission)</term><term>Infections à coronavirus (virologie)</term><term>Infections à coronavirus (épidémiologie)</term><term>Mâle</term><term>Ovis (virologie)</term><term>Phylogénie</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Réservoirs d'agents pathogènes (virologie)</term><term>Test ELISA</term><term>Zoonoses (transmission)</term><term>Zoonoses (virologie)</term><term>Zoonoses (épidémiologie)</term><term>Études transversales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>Saudi Arabia</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronavirus Infections</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Capra</term><term>Chameaux</term><term>Infections à coronavirus</term><term>Ovis</term><term>Réservoirs d'agents pathogènes</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelus</term><term>Coronavirus Infections</term><term>Disease Reservoirs</term><term>Goats</term><term>Sheep</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Arabie saoudite</term><term>Infections à coronavirus</term><term>Zoonoses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Cross-Sectional Studies</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Phylogeny</term><term>Real-Time Polymerase Chain Reaction</term><term>Whole Genome Sequencing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte d'âge moyen</term><term>Animaux</term><term>Bovins</term><term>Humains</term><term>Mâle</term><term>Phylogénie</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Test ELISA</term><term>Études transversales</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans.</p></sec><sec><title>Methods</title><p>This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014–2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed.</p></sec><sec><title>Results</title><p>The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula.</p></sec><sec><title>Conclusions</title><p>These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Assiri, A" uniqKey="Assiri A">A. Assiri</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. 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Al Rabeeah</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Arabie saoudite</li><li>République populaire de Chine</li><li>Égypte</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Kasem, Samy" sort="Kasem, Samy" uniqKey="Kasem S" first="Samy" last="Kasem">Samy Kasem</name></noRegion><name sortKey="Abu Obeida, Ali" sort="Abu Obeida, Ali" uniqKey="Abu Obeida A" first="Ali" last="Abu-Obeida">Ali Abu-Obeida</name><name sortKey="Al Doweriej, Ali" sort="Al Doweriej, Ali" uniqKey="Al Doweriej A" first="Ali" last="Al-Doweriej">Ali Al-Doweriej</name><name sortKey="Al Hufofi, Ali" sort="Al Hufofi, Ali" uniqKey="Al Hufofi A" first="Ali" last="Al-Hufofi">Ali Al-Hufofi</name><name sortKey="Al Romaihi, Ahmed" sort="Al Romaihi, Ahmed" uniqKey="Al Romaihi A" first="Ahmed" last="Al-Romaihi">Ahmed Al-Romaihi</name><name sortKey="Al Sahaf, Ali" sort="Al Sahaf, Ali" uniqKey="Al Sahaf A" first="Ali" last="Al-Sahaf">Ali Al-Sahaf</name><name sortKey="Alkarar, Ali" sort="Alkarar, Ali" uniqKey="Alkarar A" first="Ali" last="Alkarar">Ali Alkarar</name><name sortKey="Babekr, Nasereldeen" sort="Babekr, Nasereldeen" uniqKey="Babekr N" first="Nasereldeen" last="Babekr">Nasereldeen Babekr</name><name sortKey="Bayoumi, Faisal" sort="Bayoumi, Faisal" uniqKey="Bayoumi F" first="Faisal" last="Bayoumi">Faisal Bayoumi</name><name sortKey="El Harby, Nadr" sort="El Harby, Nadr" uniqKey="El Harby N" first="Nadr" last="El-Harby">Nadr El-Harby</name><name sortKey="Elfadil, Abdelhamid" sort="Elfadil, Abdelhamid" uniqKey="Elfadil A" first="Abdelhamid" last="Elfadil">Abdelhamid Elfadil</name><name sortKey="Gaafer, Albagir" sort="Gaafer, Albagir" uniqKey="Gaafer A" first="Albagir" last="Gaafer">Albagir Gaafer</name><name sortKey="Hashim, Osman" sort="Hashim, Osman" uniqKey="Hashim O" first="Osman" last="Hashim">Osman Hashim</name><name sortKey="Hussien, Raed" sort="Hussien, Raed" uniqKey="Hussien R" first="Raed" last="Hussien">Raed Hussien</name><name sortKey="Qasim, Ibraheem" sort="Qasim, Ibraheem" uniqKey="Qasim I" first="Ibraheem" last="Qasim">Ibraheem Qasim</name><name sortKey="Zaki, Ahmed" sort="Zaki, Ahmed" uniqKey="Zaki A" first="Ahmed" last="Zaki">Ahmed Zaki</name></country><country name="Égypte"><noRegion><name sortKey="Kasem, Samy" sort="Kasem, Samy" uniqKey="Kasem S" first="Samy" last="Kasem">Samy Kasem</name></noRegion></country><country name="République populaire de Chine"><noRegion><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name></noRegion><name sortKey="Chu, Daniel K W" sort="Chu, Daniel K W" uniqKey="Chu D" first="Daniel K. W." last="Chu">Daniel K. W. Chu</name><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><name sortKey="Perera, Ranawaka A P M" sort="Perera, Ranawaka A P M" uniqKey="Perera R" first="Ranawaka A. P. M." last="Perera">Ranawaka A. P. M. Perera</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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